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瑞士羅氏 |
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應用不同組裝的磷脂酰膽堿對牛精漿蛋白的隔離——結論、致謝！
來(lái)源：上海謂載 瀏覽 779 次 發(fā)布時(shí)間:2021-10-20
結論
首先，表面張力測量證明牛精漿具有非常好的表面活性，這可能與 BSP 蛋白。 二、朗繆爾薄膜法 先前被證明是相關(guān)的脂質(zhì)單層模型 公牛精子的外細胞膜 [35]，有助于表征 BSP 蛋白對脂質(zhì)膜的親和力。 BSP 蛋白是 能夠到達被磷脂覆蓋的牛精子細胞表面，這要歸功于它們與磷脂酰膽堿和 它們自身的表面活性。 我們的假設是，由于它們自己的表面，它們首先穿透精子的外葉 活動(dòng)然后他們留在那里因為與 磷脂酰膽堿。 最后，Langmuir 薄膜法也得到了 用于篩選已知的 BSP 蛋白螯合劑（如 LDL 和脂質(zhì)體）的作用。 脂質(zhì)體被證明是 與 LDL 一樣有效地防止 BSP 蛋白插入 磷脂層。 發(fā)現 LDL 和 脂質(zhì)體：0.16–0.17 mg 磷脂酰膽堿/mg BSP。 我們不 還知道脂質(zhì)體在 冷凍保存的時(shí)間尺度有一些生物學(xué)后果。 需要進(jìn)一步的研究來(lái)分析每個(gè)的表面特性 BSP 蛋白及其對膜的作用。 此外，可以測試朗繆爾薄膜以檢測 BSP 對膜的親和力隨溫度的變化 [20]。 最后，朗繆爾薄膜法是一種 用于鑒定新的螯合劑的強大篩選方法 BSP 蛋白質(zhì)。 這種方法可以適用于其他動(dòng)物物種。
致謝
作者要感謝 IMV Technologies（法國） 其財政支持，Gérard Chatagnon 的量化 精漿中的蛋白質(zhì)，凝膠電泳的 Véronique Solé，英語(yǔ)校正的 Maureen Collobert 和 Alain Sire 和 Patrice Papineau 的概念和實(shí)現 手套箱允許在受控的情況下使用 Langmuir 槽進(jìn)行工作 大氣層。
附錄 A. 補充數據
與本文相關(guān)的補充數據可以在 在線(xiàn)版本，見(jiàn) http://dx.doi.org/10.1016/j.colsurfb.2015.11。 034.
參考
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